Protein Engineering

Trimeric Redesign of a CAR-T Costimulatory Transmembrane Domain
Supervised by Dr. Emma Petley · PIs: Prof. Matt Call & A/Prof. Melissa Call
UniMelbWEHI 2026 Ongoing
Program
Redesigning a CAR-T costimulatory transmembrane domain back into its native trimeric form, screened across 120 µs+ of all-atom and coarse-grained MD with free-energy calculations, and validated by flow cytometry and degranulation assays.
protein engineering structural biology molecular dynamics
Computational Design of CrtW and CrtZ for Enhanced Astaxanthin Biosynthesis
Supervised by Dr. Yangwei Jiang · PI: Prof. Ruhong Zhou
ZJU Zhou Lab 2025 Ongoing
Code
Combining Rosetta FuncLib with protein language models to redesign the rate-limiting astaxanthin ketolase, validated in vitro at +46.7% yield.
enzyme engineering protein engineering structural biology molecular dynamics
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